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After a week, the cover can be gradually removed and the plants acclimated to stronger light and drier atmospheric conditions. You now have a collection of plants in your classroom that are genetically exactly the same. You could use these plants to carry out other experiments knowing that one common source of variation in the experiment has been eliminated. Some of these tests could include looking at plant responses to low light levels, to drought, or to saline soil conditions.
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The most important part of this activity, however, is to maintain as sterile an environment as possible. Even one fungal spore or bacterial cell that comes into contact with the growth medium will rapidly reproduce and soon completely overwhelm the small plant piece that you are trying to clone. If you wish to use plants other than cauliflower, you need to prepare two different media which contain plant hormones necessary to stimulate development of differentiated tissues. The first one should contain a cytokinin such as BAP which promotes shoot formation and the second one a rooting hormone such as NAA or store bought rooting hormone. To do this, prepare the mixture up until the end of step 2. Keeping the mixture warm so that it does not solidify, divide it equally into two pre-warmed containers.
Plant tissue culture involves excising plant tissues and growing them on nutrient media. It is used rather broadly to include several variations, such as meristem culture for propagation of virus-free plants, protoplast culture, cell suspension culture, tissue and organ culture, and anther or pollen culture for producing haploid plants. This chapter focuses on various technical aspects of plant tissue culture. A suitable explant is selected and prepared for culture, and later incubated on an appropriate nutrient medium for growth and differentiation.
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